Active Recombinant Human DYRK4 Protein, Flag-tagged

• Cat.No.: DYRK4-2992H
• Product Overview: Human DYRK4 (NP_003836.1, 1 a.a. - 520 a.a.) full-length recombinant protein with Flag tag expressed in baculovirus infected Sf21 cells.

Specification

• Description: This gene encodes an enzyme that belongs to a conserved family of serine/threonine protein kinases. Members of this dual specificity kinase family are thought to function in the regulation of cell differentiation and proliferation, survival, and in development. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Aug 2013]
• Source: Insect cells
• Species: Human
• Tag: Flag
• Bio-activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000.
  Substrate: DYRKtide.
  ATP: 100 μM.
• Molecular Mass: 61 kDa
• Purity: 60 % by SDS-PAGE/CBB staining
• Applications: Functional Study
  SDS-PAGE
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)

Gene Information

• Gene Name: DYRK4 dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4 [ Homo sapiens ]
• Official Symbol: DYRK4
• Synonyms: DYRK4; dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4; dual specificity tyrosine-phosphorylation-regulated kinase 4
• Gene ID: 8798
• mRNA Refseq: NM_003845
• Protein Refseq: NP_003836
• MIM: 609181
• UniProt ID: Q9NR20