Active Recombinant Human DNMT3A& Full Length DNMT3L Protein, N-6xHis-FLAG & N-6xHis tag

• Cat.No.: DNMT3A/DNMT3L-0294H
• Product Overview: Active Recombinant Human DNMT3A Protein(NP_783328.1)(214-912 aa) with an N-terminal 6xHis Tag and FLAG-Tag and full length DNMT3L (NP_787063.1) with an N-terminal 6xHis Tag was expressed in Sf9 cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: N-6xHis-FLAG & N-6xHis
• ProteinLength: 214-912 aa(DNMT3A)& full length(DNMT3L)
• Combining capacity: Please refer to product insert upon arrival for lot-specific concentration.
• Form: Supplied in 25 mM Hepes pH 7.5, 300 mM NaCl, 5% Glycerol, 0.04% Triton X-100, 0.2 mM TCEP and 0.2 mg/ml 3× Flag peptide.
• Bio-activity: Recombinant DNMT3A / DNMT3L complex activity assay Recombinant DNMT3A / DNMT3L complex activity was measured using DNMT Activity / Inhibition Assay, Active Motif Catalog No. 55006, for 1 hour at 37°C.
• Molecular Mass: The molecular weights of DNMT3A and DNMT3L are 83.8 kDa and 46.6 kDa, respectively.
• Purity: >95% pure by SDS-PAGE.
• Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: DNMT3A DNA (cytosine-5-)-methyltransferase 3 alpha [ Homo sapiens ]
• Official Symbol: DNMT3A
• Synonyms: DNMT3A; DNA (cytosine-5-)-methyltransferase 3 alpha; DNA (cytosine-5)-methyltransferase 3A; DNA MTase HsaIIIA; DNA cytosine methyltransferase 3A2; DNMT3A2; M.HsaIIIA
• Gene ID: 1788
• mRNA Refseq: NM_022552
• Protein Refseq: NP_072046
• MIM: 602769
• UniProt ID: Q9Y6K1