Active Recombinant Human Defensin, Beta 4A

• Cat.No.: DEFB4A-037H
• Product Overview: Recombinant human protein was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: Human
• Tag: Non
• Description: Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The a-defensins are distinguished from the beta-defensins by the pairing of their three disulfide bonds. To date, four human beta-defensins have been identified; BD-1, BD-2, BD-3 and BD-4. beta-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they are chemoattractant towards immature dendritic cells and memory T cells.
• Form: Supplied lyophilized with no additives. Reconstitute in 10 mM Acetic Acid to a concentration of 0.1 - 1.0 mg/ml.
• Purity: >98% pure by SDS PAGE and HPLC
• Sequence: Region of BD2 protein corresponding to amino acids GIGDPVTCLK SGAICHPVFC PRRYKQIGTC GLPGTKCCKK P.
• Molecular Mass: 4.3 kDa
• Applications: Numerous applications are possible with this product and should be tested by the end user under their own laboratory conditions
• Endotoxin Levels: Endotoxin level is less than 0.1 ng per ug (1 EU/ug).
• Bioactivity: Determined by its ability to chemoattract immature human dendritic cells using a concentration of 10.0-100.0 ng/ml.
• Storage: Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Gene Information

• Gene Name: DEFB4A defensin, beta 4A [ Homo sapiens ]
• Official Symbol: DEFB4A
• Synonyms: DEFB4A; SAP1; DEFB2; DEFB4; HBD-2; DEFB-2; DEFB102; BD-2; DEFB4B; hBD-2; defensin, beta 4A; beta-defensin 4A; defensin, beta 2; defensin, beta 4; OTTHUMP00000159651; skin-antimicrobial peptide 1; Beta-defensin 2
• Gene ID: 1673
• mRNA Refseq: NM_004942
• Protein Refseq: NP_004933
• MIM: 602215
• UniProt ID: O15263
• Chromosome Location: 8p23.1