Active Recombinant Human DAG1 Protein, His-tagged
Cat.No. : | DAG1-549H |
Product Overview : | Recombinant Human DAG1 (Accession # NP_004384) Met1-Val749, fused with with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived. |
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Species : | Human |
Source : | Mammalian Cells |
Tag : | His |
Protein Length : | Met1-Val749 |
Predicted N Terminal : | Gln28 predicted ( alpha subunit): No results obtained, sequencing might be blocked & Ser654 ( beta subunit) |
Form : | Lyophilized from a 0.2 µm filtered solution in PBS. |
Bio-activity : | Measured by the ability of the immobilized protein to enhance the adhesion of H4 human neuroglioma cells.The ED50 for this effect is typically 1.5 - 6.0 µg/mL. Optimal dilutions should be determined by each laboratory for each application. |
Molecular Mass : | Recombinant Human DAG1, His-tagged has a calculated MW of 67.8 kDa ( alpha subunit) & 11.3 kDa ( beta subunit). In SDS-PAGE migrates as 65-90 kDa & 20 kDa, reducing conditions. |
Purity : | >95%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
Storage : | Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC). |
Reconstitution : | Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
Gene Name | DAG1 dystroglycan 1 (dystrophin-associated glycoprotein 1) [ Homo sapiens ] |
Official Symbol | DAG1 |
Synonyms | DAG1; dystroglycan 1 (dystrophin-associated glycoprotein 1); dystroglycan; 156DAG; A3a; AGRNR; alpha dystroglycan; beta dystroglycan; DAG; dystrophin associated glycoprotein 1; MDDGC7; FLJ51254; |
Gene ID | 1605 |
mRNA Refseq | NM_001165928 |
Protein Refseq | NP_001159400 |
MIM | 128239 |
UniProt ID | Q14118 |
Chromosome Location | 3p21 |
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Not For Human Consumption!
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