Active Recombinant Human CYR61

• Cat.No.: CYR61-531H
• Product Overview: Recombinant Human CYR61 produced in E. coli is approximately 39.4 kDa, a single non-glycosylated polypeptide chain containing 357 amino acids.

Specification

• Description: Cysteine-rich angiogenic inducer 61 (Cyr61) encoded by the Cyr61 gene is a dynamically expressed, multifunctional matricellular protein and it is also a secreted, extracellular matrix (ECM)-associated signaling protein of the CCN family. Cyr61 plays essential roles in cardiovascular development during embryogenesis and regulates inflammation, wound healing and fibrogenesis in the adult. Aberrant CCN1 expression is associated with myriad pathologies, including various cancers and diseases associated with chronic inflammation. Mature human Cyr61 shares 93 % amino acid sequence identity with mouse and rat Cyr61. Cyr61 consists of four domains. There are an IGFBP domain, a VWF type C domain, a TSP type I domain, and a cysteine knot domain.
• Source: E. coli
• Species: Human
• Form: Lyophilized from a 0.2 μm filtered concentrated solution in citrate buffer solution, 300 mM NaCl, pH 3.0.
• Bio-activity: Fully biologically active when compared to standard. The ED50 determined by a cell proliferation assay using murine Balb/3T3 cells is less than 3.0 μg/ml, corresponding to a specific activity of >3.3 × 10^5IU/mg.
• Molecular Mass: 39.4 kDa
• AA Sequence: TCPAACHCPL EAPKCAPGVG LVRDGCGCCK VCAKQLNEDC SKTQPCDHTK GLECNFGASS TALKGICRAQ SEGRPCEYNS RIYQNGESFQ PNCKHQCTCI DGAVGCIPLC PQELSLPNLG CPNPRLVKVT GQCCEEWVCD EDSIKDPMED QDGLLGKELG FDASEVELTR NNELIAVGKG SSLKRLPVFG MEPRILYNPL QGQKCIVQTT SWSQCSKTCG TGISTRVTND NP
• Endotoxin: Less than 1 EU/μg of rHuCYR61 as determined by LAL method.
• Purity: >95% by SDS-PAGE and HPLC analyses.
• Usage: This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
• Storage: This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 centigrade to -70 centigrade. Avoid repeated freeze/thaw cycles.
• Reconstitution: We recommend that this vial be briefy centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 centigrade. Further dilutions should be made in appropriate buffered solutions.
• Tag: Non

Gene Information

• Gene Name: CYR61 cysteine-rich, angiogenic inducer, 61 [ Homo sapiens ]
• Official Symbol: CYR61
• Synonyms: CYR61; cysteine-rich, angiogenic inducer, 61; IGFBP10; protein CYR61; CCN1; GIG1; IBP-10; IGFBP-10; CCN family member 1; IGF-binding protein 10; cysteine-rich, anigogenic inducer, 61; cysteine-rich heparin-binding protein 61; insulin-like growth factor-binding protein 10
• Gene ID: 3491
• mRNA Refseq: NM_001554
• Protein Refseq: NP_001545
• MIM: 602369
• UniProt ID: O00622
• Chromosome Location: 1p22.3
• Pathway: Hypertrophy Model, organism-specific biosystem; Regulation of Wnt-mediated beta catenin signaling and target gene transcription, organism-specific biosystem; RhoA signaling pathway, organism-specific biosystem; amb2 Integrin signaling, organism-specific biosystem
• Function: extracellular matrix binding; heparin binding; insulin-like growth factor binding; integrin binding