Active Recombinant Human CYP46A1 Protein

• Cat.No.: CYP46A1-03H
• Product Overview: Recombinant human CYP46A1 and human CYP-reductase without tag was coexpressed in Escherichia coli.
  Amount: 0.5 nmol cytochrome P450

Specification

• Species: Human
• Source: E.coli
• Description: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein is expressed in the brain, where it converts cholesterol to 24S-hydroxycholesterol. While cholesterol cannot pass the blood-brain barrier, 24S-hydroxycholesterol can be secreted in the brain into the circulation to be returned to the liver for catabolism.
• Bio-activity: Cytochrome c Reductase Activity: 91 nmol/min/mg protein
  P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 50 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase])
  Activity: Testosterone 16β-hydroxylase
  Linear with time up to: 15 min(Determined at 15 µM substrate, using a P450 concentration of 75 pmol/ml)
  Linear with P450 up to: 100 pmol/ml(Determined at 15 µM substrate, using an incubation time of 5 min)
  Vmax: 1.1 pmol/min/pmol CYP
  Km: 15 µM
• Usage: For laboratory (research) purposes only.
• Storage: Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
• Concentration: P450 Concentration: 1.0 nmol/ml
  Protein Concentration: 10.0 mg/ml
  Specific P450 Content: 100 pmol/mg protein
• Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Gene Information

• Gene Name: CYP46A1 cytochrome P450 family 46 subfamily A member 1 [ Homo sapiens (human) ]
• Official Symbol: CYP46A1
• Synonyms: CYP46A1; cytochrome P450 family 46 subfamily A member 1; CP46; CYP46; cholesterol 24-hydroxylase; CH24H; cholesterol 24-monooxygenase; cholesterol 24S-hydroxylase; cytochrome P450 46A1; cytochrome P450, family 46, subfamily A, polypeptide 1; cytochrome P450, subfamily 46 (cholesterol 24-hydroxylase); EC 1.14.14.25
• Gene ID: 10858
• mRNA Refseq: NM_006668
• Protein Refseq: NP_006659
• MIM: 604087
• UniProt ID: Q9Y6A2