Active Recombinant Human CYP2J2 Protein

• Cat.No.: CYP2J2-47H
• Product Overview: Recombinant human CYP2J2 and human CYP-reductase without tag was coexpressed in Escherichia coli.
  Amount: 0.5 nmol cytochrome P450

Specification

• Species: Human
• Source: E.coli
• Description: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is thought to be the predominant enzyme responsible for epoxidation of endogenous arachidonic acid in cardiac tissue. Multiple transcript variants have been found for this gene.
• Bio-activity: Cytochrome c Reductase Activity: 353 nmol/min/mg protein
  P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 100 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase])
  Activity: Terfenadine C-hydroxylase
  Linear with time up to: 15 min(Determined at 1 µM substrate, using a P450 concentration of 0.125 pmol/ml)
  Linear with P450 up to: 0.4 pmol/ml(Determined at 1 µM substrate, using an incubation time of 5 min)
  Vmax: 33 pmol/min/pmol CYP
  Km: 0.02 µM
• Usage: For laboratory (research) purposes only.
• Storage: Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
• Concentration: P450 Concentration: 1.0 nmol/ml
  Protein Concentration: 10.0 mg/ml
  Specific P450 Content: 100 pmol/mg protein
• Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Gene Information

• Gene Name: CYP2J2 cytochrome P450 family 2 subfamily J member 2 [ Homo sapiens (human) ]
• Official Symbol: CYP2J2
• Synonyms: CYP2J2; cytochrome P450 family 2 subfamily J member 2; CPJ2; CYPIIJ2; cytochrome P450 2J2; albendazole monooxygenase (hydroxylating); albendazole monooxygenase (sulfoxide-forming); arachidonic acid epoxygenase; cytochrome P450, family 2, subfamily J, polypeptide 2; cytochrome P450, subfamily IIJ (arachidonic acid epoxygenase) polypeptide 2; flavoprotein-linked monooxygenase; hydroperoxy icosatetraenoate isomerase; microsomal monooxygenase; EC 1.14.14.73; EC 1.14.14.74; EC 5.4.4.7
• Gene ID: 1573
• mRNA Refseq: NM_000775
• Protein Refseq: NP_000766
• MIM: 601258
• UniProt ID: P51589