Active Recombinant Human CYLD Protein, His-tagged

• Cat.No.: CYLD-23H
• Product Overview: Recombinant human CYLD isoform 1 protein Protein contains an N-terminal 6-His tag was expressed in Spodoptera frugiperda, Sf 21.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Description: This gene is encodes a cytoplasmic protein with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains that functions as a deubiquitinating enzyme. Mutations in this gene have been associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
• Bio-activity: Recombinant Human His6-CYLD Isoform 1 is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-CYLD Isoform 1 concentration of 50-500 nM.
• Molecular Mass: 108 kDa
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
• Storage: Avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -70 centigrade as supplied. 3 months, -70 centigrade under sterile conditions after opening.
• Storage Buffer: X mg/ml (X μM) in 50 mM HEPES pH 8.0, 200 mM NaCl, 2 mM DTT
• Shipping: The product is shipped with dry ice or equivalent.

Gene Information

• Gene Name: CYLD CYLD lysine 63 deubiquitinase [ Homo sapiens (human) ]
• Official Symbol: CYLD
• Synonyms: CYLD; CYLD lysine 63 deubiquitinase; EAC; MFT; SBS; TEM; BRSS; CDMT; MFT1; CYLD1; CYLDI; USPL2; ubiquitin carboxyl-terminal hydrolase CYLD; cylindromatosis (turban tumor syndrome); deubiquitinating enzyme CYLD; probable ubiquitin carboxyl-terminal hydrolase CYLD; ubiquitin specific peptidase like 2; ubiquitin thioesterase CYLD; ubiquitin thiolesterase CYLD; ubiquitin-specific-processing protease CYLD; EC 3.4.19.12
• Gene ID: 1540
• mRNA Refseq: NM_015247
• Protein Refseq: NP_056062
• MIM: 605018
• UniProt ID: Q9NQC7