Active Recombinant Human CXCL5 protein

• Cat.No.: CXCL5-151H
• Product Overview: Recombinant Human CXCL5 protein was expressed in HEK293T.

Specification

• Species: Human
• Source: HEK293
• Description: Epithelial cell­derived neutrophil­activating peptide (ENA­78) also known as C-X-C motif chemokine 5 (CXCL5), is a small cytokine belonging to the CXC chemokine family. It is produced following stimulation of cells with the inflammatory cytokines interleukin-1 or tumor necrosis factor-alpha. Expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon, IFN-γ. This chemokine stimulates the chemotaxis of neutrophils possessing angiogenic properties. Full length CXCL5 (78 aa) is trimmed at the N­terminal end by cathepsin G and chymotrypsin to ENA-74 (74 aa) and ENA-70 (70aa), with the shortened forms showing increased potency relative to full length CXCL5. CXCL5can signal through the CXCR2 receptor.
• Form: Lyophilized after extensive dialysis against PBS.
• Bio-activity: The EC50 value of human ENA-78/CXCL5 on Ca2+ mobilization assay in CHO-K1/ Gα15/hCXCR2 cells (human Gα15 and human CXCR2 stably expressed in CHO-K1 cells) is less than 200.0 ng/ml.
• Molecular Mass: ~8.5 kDa
• Endotoxin: < 0.2 EU/μg of protein by gel clotting method.
• Purity: > 98%
• Storage: Upon receiving, this product remains stable for up to 6 months at lower than -70 centigrade. Upon reconstitution, the product should be stable for up to 1 week at 4 centigrade or up to 3 months at -20 centigrade. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.

Gene Information

• Gene Name: CXCL5 C-X-C motif chemokine ligand 5 [ Homo sapiens (human) ]
• Official Symbol: CXCL5
• Synonyms: C-X-C motif chemokine 5; ENA-78; Epithelial-derived neutrophil-activating protein 78; Neutrophil-activating peptide ENA-78; Small-inducible cytokine B5; ENA78; SCYB5
• Gene ID: 6374
• mRNA Refseq: NM_002994
• Protein Refseq: NP_002985
• MIM: 600324
• UniProt ID: P42830