Active Recombinant Human CSF2 Protein

• Cat.No.: CSF2-135H
• Product Overview: Recombinant Human Granulocyte-Macrophage Colony-Stimulating Factor is produced by our E.coli expression system and the target gene encoding Ala18-Glu144 is expressed.

Specification

• Species: Human
• Source: E.coli
• Protein Length: Ala18-Glu144
• Description: Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic, monocytes/ macrophages and eosinophils. GM-CSF has a functional role on non-hematopoitic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF can also stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines.
• Form: Lyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, 5% Mannitol, pH 7.2.
• Bio-activity: Measured by the dose-dependent stimulation of human TF-1 cell proliferation. ED50 is less than 0.1 ng/ml. Specific Activity of 1.0 x 10^7 IU/ mg.
• AA Sequence: MAPARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQEPTCLQTRLELYKQGL RGSLTKLKGPLTMMAS HYKQHCPPTPETSCATQIITFESFKENLKDFLLVIPFDCWEPVQE
• Endotoxin: Less than 0.1 ng/ug (1 EU/ug).
• Purity: >95%
• Storage: Lyophilized protein should be stored at < -20 centigrade, though stable at room temperature for 3 weeks.
  Reconstituted protein solution can be stored at 4-7 centigrade for 2-7 days.
  Aliquots of reconstituted samples are stable at < -20 centigrade for 3 months.
• Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
  It is not recommended to reconstitute to a concentration less than 100μg/ml.
  Dissolve the lyophilized protein in distilled water.
  Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Quality Statement: Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Shipping: The product is shipped at ambient temperature.
  Upon receipt, store it immediately at the temperature listed below.

Gene Information

• Gene Name: CSF2 colony stimulating factor 2 [ Homo sapiens (human) ]
• Official Symbol: CSF2
• Synonyms: Granulocyte-Macrophage Colony-Stimulating Factor; GM-CSF; Colony-Stimulating Factor; CSF; Molgramostin; Sargramostim; GMCSF
• Gene ID: 1437
• mRNA Refseq: NM_000758.4
• Protein Refseq: NP_000749.2
• MIM: 138960
• UniProt ID: P04141