Active Recombinant Human CSF1 Protein (Glu33-Ser190), GMP Grade, Animal-Free

• Cat.No.: CSF1-18HG
• Product Overview: GMP recombinant human M-CSF protein (Glu33-Ser190) with an N-terminal Met without tag was expressed in E. coli, produced using non-animal reagents in an Animal-Free laboratory and manufactured and tested under cGMP guidelines.

Specification

• Species: Human
• Source: E.coli
• Protein Length: Glu33-Ser190
• Description: The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]
• Form: Disulfide-linked homodimer
• Bio-activity: Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 0.5-1.5 ng/mL.
  The specific activity of Recombinant Human M-CSF is >6.0 x 107 IU/mg, which is calibrated against the human M-CSF WHO International Standard.
• Molecular Mass: Predicted Molecular Mass: 18.5 kDa (monomer)
  SDS-PAGE: 37 kDa, non-reducing conditions
• N-terminal Sequence Analysis: Met-Glu-Glu-Val-Ser-Glu-Try-(Cys)-Ser-His
• Endotoxin: <0.01 EU/ μg of the protein by the LAL method.
• Purity: >97%, by SDS-PAGE with silver staining, under reducing conditions.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  A minimum of 12 months when stored at ≤ -20 centigrade as supplied. Refer to lot specific COA for the Use by Date.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, ≤ -20 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 50-500 μg/mL in PBS.
• Shipping: The product is shipped with polar packs.

Gene Information

• Gene Name: CSF1 colony stimulating factor 1 (macrophage) [ Homo sapiens ]
• Official Symbol: CSF1
• Synonyms: CSF1; colony stimulating factor 1 (macrophage); macrophage colony-stimulating factor 1; M CSF; MCSF; MGC31930; lanimostim; CSF-1
• Gene ID: 1435
• mRNA Refseq: NM_172211
• Protein Refseq: NP_757350
• MIM: 120420
• UniProt ID: P09603