Active Recombinant Human CREBBP, GST-tagged

• Cat.No.: CREBBP-192H
• Product Overview: Recombinant human KAT3A (CREBBP) (1319-1710) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: KAT3A has intrinsic histone acetyltransferase activity that acts as a scaffold to stabilize additional protein interactions with the transcription complex and acetylates both histone and non-histone proteins. KAT3A (CREBBP) expressed as a nuclear protein that binds to cAMP-response element binding protein (CREB) and involved in the transcriptional coactivation of many different transcription factors. KAT3A plays a main role in embryonic development, growth control, and homeostasis by coupling chromatin remodeling to transcription factor recognition. KAT3A also plays a critical role in the transmission of inductive signals from cell surface receptors to the transcriptional apparatus.
• Source: Sf9 insect cells using baculovirus
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: The specific activity of KAT3A (CREBBP) was determined to be 32 nmol /min/mg
• Molecular Mass: ~72 kDa
• Purity: >90% by densitometry
• Applications: Acetyltransferase Assay
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μl
• Protein length: 1319-1710 a.a.

Gene Information

• Gene Name: CREBBP CREB binding protein [ Homo sapiens ]
• Official Symbol: CREBBP
• Synonyms: CREBBP; CREB binding protein; RSTS, Rubinstein Taybi syndrome; CREB-binding protein; CBP; KAT3A; RTS; RSTS
• Gene ID: 1387
• mRNA Refseq: NM_001079846
• Protein Refseq: NP_001073315
• MIM: 600140
• UniProt ID: Q92793
• Chromosome Location: 16p13.3
• Pathway: Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; Angiogenesis, organism-specific biosystem; BMAL1:CLOCK/NPAS2 Activates Circadian Expression, organism-specific biosystem; C-MYB transcription factor network, organism-specific biosystem; Cell cycle, organism-specific biosystem
• Function: DNA binding; MyoD binding; RNA polymerase II activating transcription factor binding; RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription; RNA polymerase II transcription coactivator activity; RNA polymerase II transcription factor binding; RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription; SMAD binding; acetyltransferase activity; chromatin binding; core promoter proximal region sequence-specific DNA binding; histone acetyltransferase activity; histone acetyltransferase activity; metal ion binding; p53 binding; peroxisome proliferator activated receptor binding; protein binding; sequence-specific DNA binding transcription factor activity; signal transducer activity; transcription coactivator activity; contributes_to transcription coactivator activity; transcription coactivator activity; transcription coactivator activity; transcription factor binding; transferase activity; zinc ion binding