Active Recombinant Mouse Crebbp, His-tagged

• Cat.No.: Crebbp-168M
• Product Overview: Mouse CREB-binding protein fused at the C-terminus to a His-tag.

Specification

• Species: Mouse
• Source: Sf21 Cells
• Tag: His
• Description: CREB-binding protein acetylates histones, giving a specific tag for transcriptional activation. It also acetylates non-histone proteins, like NCOA3 and FOXO1. It binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. Acts as a coactivator of ALX1 in the presence of EP300.
• Form: Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 0.2% NP-40, 50-100mM imidazole and 10% glycerol.
• Bio-activity: ~50-100ng are required for standard HAT assay.
• Purity: ≥95% (SDS-PAGE)
• Stability: Stable for at least 6 months after receipt when stored at -80°C.
• Storage: Short Term Storage: -20°C; Long Term Storage: -80°C. After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.
• Concentration: Lot dependent (0.2-0.5mg/ml)

Gene Information

• Gene Name: Crebbp CREB binding protein [ Mus musculus ]
• Official Symbol: Crebbp
• Synonyms: CREBBP; CREB binding protein; CREB-binding protein; CBP; KAT3A; AW558298; CBP/p300; p300/CBP
• Gene ID: 12914
• mRNA Refseq: NM_001025432
• Protein Refseq: NP_001020603
• Pathway: Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, conserved biosystem; Cell cycle signaling pathway, organism-specific biosystem; Developmental Biology, organism-specific biosystem
• Function: DNA binding; MyoD binding; RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription; RNA polymerase II transcription factor binding; RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription; SMAD binding; acetyltransferase activity; acetyltransferase activity; activating transcription factor binding; chromatin binding; core promoter proximal region sequence-specific DNA binding; histone acetyltransferase activity; histone acetyltransferase activity; metal ion binding; p53 binding; peroxisome proliferator activated receptor binding; protein binding; contributes_to sequence-specific DNA binding transcription factor activity; transcription coactivator activity; transcription coactivator activity; transcription cofactor activity; transcription factor binding; transcription factor binding; transferase activity; zinc ion binding