Active Recombinant Human CPM, His-tagged

• Cat.No.: CPM-175H
• Product Overview: Recombinant Human CPM /Carboxypeptidase M Protein (rh CPM /Carboxypeptidase M) Leu 18 - His 422 (Accession # AAH22276) was produced in human 293 cells (HEK293).

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 18-422 a.a.
• Description: Carboxypeptidase M (CPM) belongs to the peptidase M14 family. CPM specifically removes C-terminal basic residues (Arg or Lys) from peptides and proteins, and the activity is inhibited by O-phenanthroline and MGTA and activated by cobalt. The amino acid sequence of CPM indicated that the C-terminal hydrophobic region might be a signal for membrane attachment via a glycosylphosphatidylinositol (GPI) anchor. CPM is involved in peptide metabolism on both the cell surface and in extracellular fluids. CPM functions not only as a protease but also as a binding partner in cell-surface protein-protein interactions.
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM MES, pH 6.5 with 100 mM NaCl. Normally Mannitol or Trehalose are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to release Larginine from BenzoylAlaArg, with detection of the arginine amino group by ophthaldialdehyde.The specific activity is >40,000 pmoles/min/μg.
• Molecular Mass: rh CPM /Carboxypeptidase M is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 47.1 kDa. The predicted N-terminus is Leu 18. DTT-reduced Protein migrates as 49 kDa in SDS-PAGE due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg of the rh CPM /Carboxypeptidase M by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).
• Reconstitution: See Certificate of Analysis for reconstitution instructions and specific concentrations.

Gene Information

• Gene Name: CPM carboxypeptidase M [ Homo sapiens ]
• Official Symbol: CPM
• Synonyms: CPM; carboxypeptidase M; renal carboxypeptidase; urinary carboxypeptidase B
• Gene ID: 1368
• mRNA Refseq: NM_001874
• Protein Refseq: NP_001865
• MIM: 114860
• UniProt ID: P14384
• Chromosome Location: 12q15
• Function: carboxypeptidase activity; metal ion binding; metallocarboxypeptidase activity; metallopeptidase activity; peptidase activity; zinc ion binding