Active Recombinant Human Colony Stimulating Factor 1 (Macrophage)

• Cat.No.: CSF1-167H
• Product Overview: Recombinant Human interleukin 4 encoding the N-terminal 190 amino acids of the human Macrophage Colony-Stimulating Factor 1 (M-CSF) protein and was expressed in modifiedhuman 293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: M-CSF (macrophage colony stimulating factor), also known as colony stimulating factor-1 (CSF-1), is a homodimeric glycoprotein the subunits of which are linked by disulfide bonds. Different splice variants of M-CSF have been found which differ at the C-terminus.
• Amino Acid Sequence: EEVSEYCSHMIGSGH LQSLQRLIDSQME TSCQITFEFV DQEQLKDPV CYLKK AFL LV QD I ME DTMR FRDNTPNAIA IVQLQELSLR LK SCFTK DYEEH DKACV RTF YE TPLQL LE K V KNVFNETKNLLDKDWNIFSKNCNNSFAECSSQDVVTKPDCN.
• Molecular Mass: Under reducing conditionsM-CSFmigrates as two bands between 20 and 35 kDa in SDS-PAGE due to post-translational modifications, in particular glycosylation. This compares with unmodified M-CSF that has a predicted molecular mass of 18.4 kDa.
• PI: M-CSF separates into a number of isoforms with a pI between 3.5 and 5 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified M-CSF that has a predicted pI of 4.8.
• % Carbohydrate: Purified M-CSF consists of 0–50% carbohydrate by weight.
• Glycosylation: M-CSF contains N- and O-linked oligosaccharides.
• Purity: >95%, as determined by SDS-PAGE and visualised by Coomassie Brilliant Blue.
• Formulation: When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
• Reconstitution: It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
• Storage: Lyophilised products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
• Activity: The ED₅₀of M-CSF is typically 5–15 ng /ml as measured in a cell proliferation assay using the M-CSF dependent mouse monocytic mNFS-60 cell line.

Gene Information

• Gene Name: CSF1 colony stimulating factor 1 (macrophage) [ Homo sapiens ]
• Synonyms: CSF1; colony stimulating factor 1 (macrophage); MCSF; MGC31930; OTTHUMP00000013363; OTTHUMP00000013364; colony stimulating factor 1; macrophage colony stimulating factor; OTTHUMP00000013362
• Gene ID: 1435
• mRNA Refseq: NM_000757
• Protein Refseq: NP_000748
• MIM: 120420
• UniProt ID: P09603
• Chromosome Location: 1q21-p13
• Pathway: Cytokine-cytokine receptor interaction; Hematopoietic cell lineage
• Function: cytokine activity; growth factor activity; protein binding; macrophage colony stimulating factor receptor binding; protein homodimerization activity