Active Recombinant Human COL23A1 Protein, His-tagged

• Cat.No.: COL23A1-855H
• Product Overview: Recombinant Human COL23A1 (Accession # NP_775736) Glu111-Lys540, fused with an N-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: His
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of SW1353 human chondrosarcoma cells.When 5 x 10^4 cells/well are added to rhCOL23A1 coated plates (10 µg/mL, 100 µL/well), approximately 60% - 80% will adhere after 60 minutes at 37° C.
• Molecular Mass: Recombinant Human COL23A1, His-tagged has a calculated MW of 41.9 kDa. In SDS-PAGE migrates as 55-65 kDa, reducing conditions.
• Protein length: Glu111-Lys540
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: COL23A1 collagen, type XXIII, alpha 1 [ Homo sapiens ]
• Official Symbol: COL23A1
• Synonyms: COL23A1; collagen, type XXIII, alpha 1; collagen alpha-1(XXIII) chain; DKFZp434K0621; procollagen, type XXIII, alpha 1
• Gene ID: 91522
• mRNA Refseq: NM_173465
• Protein Refseq: NP_775736
• MIM: 610043
• UniProt ID: Q86Y22
• Chromosome Location: 5q35.3