Active Recombinant Human CLDN18.2 Full Length Transmembrane protein, Flag tag(Synthetic Nanodisc)

• Cat.No.: CLDN18-396H
• Product Overview: Active Recombinant Human CLDN18.2 Full Length Transmembrane protein(P56856), fused with Flag tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Flag
• Protein Length: Full Length
• Form: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Do not use solvents with pH lower than 6.5 in subsequent experiments.
• Bio-activity: Elisa plates were added with Flag Tag CLDN18.2-Nanodisc (0.2μg/per well) on an anti-Flag monoclonal antibody pre-coated (0.2μg/per well) plate. Serial diluted anti- CLDN18.2 monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti- CLDN18.2 monoclonal antibody binding with CLDN18.2-Nanodisc is 593.6ng/ml.
• Molecular Mass: The human full length CLDN18.2 Protein has a MW of 27.5 kDa
• Storage: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Full Length: Full L.