Active Recombinant Human Chromosome 6 Open Reading Frame 25, Fc-tagged

• Cat.No.: C6orf25-5603H
• Product Overview: Recombinant Human C6orf25protein was expressed in CHO with the Fc part of human IgG1 Pro100-Lys330(C-terminus) and Asn18-Gln142 (N-terminus).

Specification

• Species: Human
• Source: CHO
• Tag: Fc
• Protein Length: 18-142 a.a.
• Description: C6orf25 is a type Itransmembrane glycoprotein in the immunoglobulin superfamily that is encodedwithin the human class III major histocompatibility complex. It is expressedmainly on platelets, and has also been detected on megakaryocytes and thehuman cell line K562. Crosslinking of C6orf25 inhibits agoniststimulatedplatelet aggregation and activation. The 241 amino acid (aa) human C6orf25isotype B (G6bB) contains a 17 aa signal sequence, a 125 aa extracellulardomain with one Vtype Ig-like domain and one N-linked glycosylation site, a21 aa transmembrane sequence and a 78 aa cytoplasmic sequence with two ITIMinhibitory motifs and a SH3 binding site.
• Form: Lyophilized from a0.2 μm filtered solution in PBS.
• N-terminalSequence: Asn18
• Molecular Weight: 40.0 kDa
• Activity: Measuredby its binding ability in a functional ELISA. When 1 μg/mL of rhC6orf25/Fc is captured by a goat antihuman IgG Fc coated plate,the concentration of biotinylated heparin that produces 50% of the optimal bindingresponse is found to be approximately 10-40 ng/mL.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• SDS-PAGE: 45-55kDa, reducing conditions
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstituteat 100 μg/mL in PBS
• Storage: Use a manualdefrost freezer and avoid repeated freeze-thaw cycles. 12 months from date ofreceipt, -20 to -70°C as supplied. 1 month, 2 to 8 °C under sterile conditionsafter reconstitution. 3 months, -20 to -70°C under steril conditions after reconstitution.

Gene Information

• Gene Name: C6orf25 chromosome 6 openreading frame 25 [ Homo sapiens ]
• Official Symbol: C6orf25
• Synonyms: C6orf25; chromosome6 open reading frame 25; G6b; NG31; MGC142279, MGC142281; protein G6b;immunoglobulin receptor; OTTHUMP00000029171; OTTHUMP00000029172; OTTHUMP00000029173;OTTHUMP00000029176; OTTHUMP00000029177; OTTHUMP00000215228;OTTHUMP00000215230
• Gene ID: 80739
• mRNA Refseq: NM_025260
• Protein Refseq: NP_079536
• MIM: 606520
• UniProt ID: O95866
• Chromosome Location: 6p21
• Function: heparin binding;receptor activity