Active Recombinant Human CERK, Flag-tagged

• Cat.No.: CERK-508H
• Product Overview: Recombinant Human CERK (1-537(end)), fused with C-terminal Flag tag, was expressed in baculovirus expression system.

Specification

• Species: Human
• Source: Insect Cells
• Tag: Flag
• Protein Length: 1-537 a.a.
• Description: CERK converts ceramide to ceramide 1-phosphate (C1P), a sphingolipid metabolite. Both CERK and C1P have been implicated in various cellular processes, including proliferation, apoptosis, phagocytosis, and inflammation.
• Form: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 40 ug/ml Flag peptide, 20% glycerol and 3 mM DTT.
• Bio-activity: 142 pmole/min/μg. Assay conditions: CERK activity was measured at 37 oC for 25 min in a buffer (20 mM HEPES pH7.2, 80 mM KCl, 3 mM CaCl2, 10 mM MgCl2, 2 mM DTT, 5 μM ATP) using a μ-Octylglucoside mixed micellar assay with 100 μM Ceramide. ATP reduction was detected using Kinase-Glo® Luminescent Kinase Assay Platform (Promega, Madison, WI).
• Molecular Mass: 60 kDa
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Stability: >6 months at –80°C

Gene Information

• Gene Name: CERK ceramide kinase [ Homo sapiens ]
• Official Symbol: CERK
• Synonyms: CERK; LK4; hCERK; dA59H18.2; dA59H18.3; ceramide kinase; lipid kinase 4; lipid kinase LK4; acylsphingosine kinase; NP_073603.2; EC 2.7.1.138
• Gene ID: 64781
• mRNA Refseq: NM_022766
• Protein Refseq: NP_073603
• MIM: 610307
• UniProt ID: Q8TCT0
• Chromosome Location: 22q13.31
• Pathway: Glycosphingolipid metabolism; Integrated Breast Cancer Pathway; Sphingolipid Metabolism
• Function: ATP binding; NAD+ kinase activity; ceramide kinase activity