Active Recombinant Human CDK8/CCNC, GST&His-tagged

• Cat.No.: CDK8/CCNC-256H
• Product Overview: Coexpression of human CDK8, amino acids M1-Y464 (as in GenBank entry NM_001260), N-terminal GST-HIS6 fusion protein with a Thrombin cleavage site and human CycC, amino acids M1-S283 (as in GenBank entry NM_005190.3), N-terminally fused to HIS6-Thrombincleavage site.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST&His
• Protein Length: 1-464;1-283 a.a.
• Description: The Cdks are serine/threonine protein kinases which are predominantly involved in the progression of the cell cycle. Subsequently, CDK-cyclin complexes (CDK7, CDK8 and CDK9) were also identified as regulators of transcription. CDK8/CycC is part of the RNA polymerase II holoenzyme complex and phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. On the other hand, CDK8/CycC phosphorylates cyclin H, a component of the CDK activating kinase (CAK), and represses activated transcription by modulation of TFIIH activity.
• Bio-activity: 17.000 pmol/mg x min
• Molecular Mass: Theoretical MW MW GST-CDK8: 83.0 kDaTheoretical MW His-CyC: 38.0 kDa
• Concentration: 0.43 mg/ml (Bradford method using BSA as standard protein)
• Storage Buffer: 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 20% glycerol

Gene Information

• Gene Name: CDK8 cyclin-dependent kinase 8 [ Homo sapiens (human) ]
• Official Symbol: CDK8
• Synonyms: cyclin-dependent kinase 8; K35; MGC126074; MGC126075; protein kinase K35; CDK8 protein kinase; cell division protein kinase 8; EC 2.7.11.22,EC 2.7.11.23; Protein kinase K35; CDK8; OTTHUMP00000018158; Mediator complex subunit CDK8; Mediator of RNA polymerase II transcription subunit CDK8
• Gene ID: 1024
• mRNA Refseq: NM_001260
• Protein Refseq: NP_001251
• MIM: 603184
• UniProt ID: P49336
• Chromosome Location: 13q12
• Pathway: Constitutive Signaling by NOTCH1 HD+PEST Domain Mutants; Developmental Biology; FBXW7 Mutants and NOTCH1 in Cancer
• Function: ATP binding; RNA polymerase II carboxy-terminal domain kinase activity; cyclin-dependent protein serine/threonine kinase activity