Active Recombinant Human CDK5, GST-tagged

• Cat.No.: CDK5-815H
• Product Overview: Human CDK5 (NM_004935|NM_003885) full-length recombinant protein with GST tag expressed in SF9 cells.

Specification

• Species: Human
• Source: Human
• Tag: GST
• Description: Recently Cdk5 has emerged as an essential kinase in sensory pathways. Recent reports of Pareek et al. from NIH suggest its necessity in pain signaling. CDK5 is required for proper development of the brain and to be activated, CDK5 must associate with CDK5R1 or CDK5R2. Unlike other cyclin dependent kinases, CDK5 does not also require phosphorylation on the T loop so that binding with the activator is sufficient to activate the kinase.
• Theoretical MW (kDa): 59, 49
• Preparation Method: Insect cell (SF9) expression system
• Purity: > 90% by densitometry
• Activity: The specific activity was determined to be 686 nmol /min/mg as per Activity Assay Protocol.
• Application: SDS-PAGE
• Storage Buffer: In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).
• Storage: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
• Unitprot ID: Q00535

Gene Information

• Gene Name: CDK5 cyclin-dependent kinase 5 [ Homo sapiens ]
• Official Symbol: CDK5
• Synonyms: CDK5; cyclin-dependent kinase 5; PSSALRE; OTTHUMP00000212917; OTTHUMP00000212939; TPKII catalytic subunit; protein kinase CDK5 splicing; cell division protein kinase 5; EC 2.7.11.22; serine/threonine-protein kinase PSSALRE; tau protein kinase II catalytic subunit SDS-PAGE Stained with Cooma-ssie Blue.
• Gene ID: 1020
• mRNA Refseq: NM_004935
• Protein Refseq: NP_004926
• MIM: 123831
• Chromosome Location: 7q36
• Pathway: Alzheimer"s disease; Axon guidance; CRMPs in Sema3A signaling; DARPP-32 events; EPHA forward signaling; Factors involved in megakaryocyte development and platelet production; Glucocorticoid receptor regulatory; networkHemostasis; IL-6 Signaling Pathway; Lissencephaly gene (LIS1) in neuronal migration and development; Nicotine Activity on Dopaminergic Neurons; Opioid Signalling; Reelin signaling pathway; Semaphorin interactions; Trk receptor signaling mediated by the MAPK pathway
• Function: ATP binding; ErbB-2 class receptor binding; acetylcholine receptor activator activity; cyclin-dependent protein kinase activity; kinase activity; nucleotide binding; p53 binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; tau-protein kinase activity

Q&As

Is CDK5 derived from human fetal cell lines?

The CDK5 is expressed from HEK293 host, which is a mammalian cells system.