Active Recombinant Human CDK2/CCNE2 protein, GST-tagged

• Cat.No.: CDK2-CCNE2-311H
• Product Overview: Recombinant full-length human CDK2 and CCNE2 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.

Specification

• Description: CDK2 is a member of the Cyclin-Dependent Kinase family that is ubiquitously expressed. CDK2 is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. CDK2 associates with and is regulated by the regulatory subunits of the complex including Cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). CDK2 phosphorylates multiple cellular substrates including SMAD3 and FOXO1. Phosphorylation of FOXO1 leads to its inhibition.
• Source: Insect cell
• Species: Human
• Tag: GST
• Form: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol
• Bio-activity: The specific activity of CDK2/CyclinE2 was determined to be 60nmol/min/mg as per activity protocol.
• Molecular Mass: CDK2 ~58 kDa and CCNE2 ~72 kDa
• Purity: The purity of CDK2/CCNE2 was determined to be >90% by densitometry.
• Applications: Kinase Assay; WB
• Stability: 1 year at -70 centigrade
• Storage: Store product at -70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μL

Gene Information

• Gene Name: cyclin dependent kinase 2 [ Homo sapiens (human) ]; cyclin E2 [ Homo sapiens (human) ]
• Official Symbol: CDK2; CCNE2
• Synonyms: CDKN2; p33; CDK2; CCNE2; CyclinE2
• Gene ID: 1017; 9134
• mRNA Refseq: NM_001798; NM_057749
• Protein Refseq: NP_001789; NP_001228
• MIM: 116953; 603775
• UniProt ID: P24941; P24864