Active Recombinant Human CDK14/CyclinY, GST-tagged

• Cat.No.: CDK14-116H
• Product Overview: Recombinant human full-length PFTK1 (CDK14) and Cyclin Y (2-end) were co-expressed by baculovirus in Sf9 insect cells using N-terminal GST tags.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Description: PFTK1 is a member of the CDC2 - related protein kinase family which is expressed primarily in the postnatal and adult nervous system. PFTK1 is expressed at high levels in brain, pancreas, kidney, heart, testis, and ovary. PFTK1 amino acid residue ser119 is required for its interaction with all four 14-3-3 isoforms, while ser120 may contribute slightly to PFTK1 binding. PFTK1 acts as a cyclin-dependent kinase that regulates cell cycle progression and cell proliferation. Overexpression of PFTK1 predicts resistance to chemotherapy in patients with oesophageal squamous cell carcinoma.
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: The specific activity of PFTK1 (CDK14)/CyclinY was determined to be 2.5 nmol /min/mg
• Molecular Mass: PFTK1 (CDK14) ~72 kDa and Cyclin Y ~64 kDa
• Purity: >90% by densitometry
• Applications: Kinase Assay, Western Blot
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μl

Gene Information

• Gene Name: CDK14 cyclin-dependent kinase 14 [ Homo sapiens ]
• Official Symbol: CDK14
• Synonyms: CDK14; cyclin-dependent kinase 14; PFTAIRE protein kinase 1 , PFTK1; PFTAIRE1; hPFTAIRE1; PFTAIRE protein kinase 1; cell division protein kinase 14; serine/threonine-protein kinase PFTAIRE-1; PFTK1; KIAA0834
• Gene ID: 5218
• mRNA Refseq: NM_012395
• Protein Refseq: NP_036527
• MIM: 610679
• UniProt ID: O94921
• Chromosome Location: 7q21-q22
• Pathway: Transcriptional misregulation in cancer, organism-specific biosystem; Transcriptional misregulation in cancer, conserved biosystem
• Function: ATP binding; cyclin binding; cyclin-dependent protein kinase activity; nucleotide binding; protein binding