Active Recombinant Human CD8A&CD8B protein, His/FLAG-tagged

• Cat.No.: CD8A&CD8B-211H
• Product Overview: Recombinant Human CD8A(Ser22-Asp182) fused with His tag at C-terminal and CD8B(Asn19-Pro170) fused with FLAG tag at N-terminal was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Flag&His
• Protein Length: 22-182 a.a.
• Description: CD8, also known as Ly2 or Leu2, is a heterodimeric glycoprotein (alpha and beta subunits) that functions in conjunction with the T cell receptor in the recognition of MHC class I/peptide complexes . CD8 alpha is expressed on double positive (CD4+ CD8+) thymocytes and mature CD8+ cytolytic T cells (CTL), intraepithelial lymphocytes (IEL), some gamma δ T cells, subsets of thymic and splenic dendritic cells (DC), and peritoneal mast cells. It can form disulfide linked homodimers or heterodimers with CD8 beta. Thymic CD8+ DC express primarily alpha beta heterodimers, while splenic CD8+ DC primarily express alpha alpha homodimers. CD8 alpha + DC can present viral antigenic peptides in complex with MHC I and prime CTL responses. The approximately 35 kDa mature mouse CD8 alpha consists of a 169 amino acid (aa) extracellular domain (ECD) with one Iglike domain, a 21 aa transmembrane segment, and a 30 aa cytoplasmic domain. Within the ECD, mouse CD8 alpha shares 49% and 64% aa sequence identity with human and rat CD8 alpha, respectively.
• Predicted N Terminal: Ser22 (CD8 alpha ) & Asn19 (CD8 beta )
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured by its ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. Bechard, D. et al. (2001) J. Immunol. 167:3099. The ED50 for this effect is 0.8-4 μg/mL.
• Molecular Mass: Predicted Molecular Mass: 26 (CD8 alpha ) & 26 (CD8 beta ) kDa
  SDS-PAGE: 32-43 kDa, reducing conditions
• Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.

Gene Information

• Gene Name: CD8A CD8a molecule [ Homo sapiens ]
• Official Symbol: CD8A
• Synonyms: CD8; MAL; p32; Leu2
• Gene ID: 925
• mRNA Refseq: NM_001145873
• Protein Refseq: NP_001139345
• MIM: 186910
• UniProt ID: P01732