Active Recombinant Human CD80 Protein, Fc & Avi-tagged, Biotinylated

• Cat.No.: CD80-583H
• Product Overview: Biotinylated Recombinant Human CD80 Protein (Val 35 - Asn 242) (NP_005182.1), fused with a human IgG1 Fc tag at the C-terminus, followed by a Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&Fc
• Protein Length: Val 35 - Asn 242
• Predicted N Terminal: Val 35
• Form: Lyophilized from sterile 50 mM tris, 100 mM glycine, pH7.5, 10% trehalose.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Human CTLA-4, Fc Tag at 5μg/mL (100 μL/well) can bind Biotinylated Human B7-1, Fc Tag with a linear range of 0.6-9.8 ng/mL.
• Molecular Mass: Recombinant Human CD80 Protein has a calculated MW of 52.3 kDa. As a result of glycosylation, The protein migrates as 65-95 kDa under reducing (R) condition and 130-160 kDa under non-reducing (NR) condition on SDS-PAGE gel.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: CD80 CD80 molecule [ Homo sapiens ]
• Official Symbol: CD80
• Synonyms: CD80; CD80 molecule; CD28LG, CD28LG1, CD80 antigen (CD28 antigen ligand 1, B7 1 antigen) , CD80 molecule; T-lymphocyte activation antigen CD80; B lymphocyte activation antigen B7; B7 1; B7.1; activation B7-1 antigen; costimulatory factor CD80; CTLA-4 counter-receptor B7.1; B-lymphocyte activation antigen B7; costimulatory molecule variant IgV-CD80; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); B7; BB1; B7-1; LAB7; CD28LG; CD28LG1
• Gene ID: 941
• mRNA Refseq: NM_005191
• Protein Refseq: NP_005182
• MIM: 112203
• UniProt ID: P33681
• Chromosome Location: 3q13.3-q21
• Pathway: Adaptive Immune System, organism-specific biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem; CD28 co-stimulation, organism-specific biosystem; CD28 dependent PI3K/Akt signaling, organism-specific biosystem
• Function: coreceptor activity; protein binding; receptor activity