Active Recombinant Human CD70 Protein, HA/GCN4-IZ-tagged

• Cat.No.: CD70-218H
• Product Overview: Recombinant Human CD27 Ligand Protein (Gln39-Pro193) with HA (YPYDVPDYA) tag and GCN4-IZ tag at N-terminus was expressed in human embryonic kidney cell.

Specification

• Species: Human
• Source: HEK293
• Tag: HA
• Protein Length: 39-193 a.a.
• Description: The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T and B lymphocytes. It induces proliferation of costimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin sythesis.
• Form: Noncovalently-linked homotrimer
• Bio-activity: Measured by its ability to induce IL-8 secretion in HT1080 human fibrosarcoma cells transfected with human CD27. The ED50 for this effect is 5-25 ng/mL in the presence of Mouse Anti-Hemagglutinin/HA Peptide Monoclonal Antibody.
• Molecular Mass: 23 kDa
• N-terminal Sequence Analysis: Tyr
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: > 95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 200 μg/mL in PBS.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: CD70 CD70 molecule [ Homo sapiens (human) ]
• Official Symbol: CD70
• Synonyms: CD70; CD70 molecule; CD27L; LPFS3; CD27-L; CD27LG; TNFSF7; TNLG8A; CD70 antigen; CD27 ligand; Ki-24 antigen; surface antigen CD70; tumor necrosis factor ligand 8A; tumor necrosis factor ligand superfamily member 7
• Gene ID: 970
• mRNA Refseq: NM_001252
• Protein Refseq: NP_001243
• MIM: 602840
• UniProt ID: P32970