Active Recombinant Human CD48 protein(Met1-Ser220), His&Avi-tagged, Biotinylated

• Cat.No.: CD48-3255H
• Product Overview: Biotinylated Recombinant Human CD48 (NP_001769.2) (Met1-Ser220) was expressed in HEK293 with a C-terminal polyhistidine tag followed by an AVI tag.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His&Avi
• Protein length: Met1-Ser220
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized Human 2B4/CD244 (hFc Tag) at 2 μg/mL (100 μL/well) can bind Human CD48 (His & AVI Tag), Biotinylated, the EC50 is 120-520 ng/mL.
• Molecular Mass: The recombinant human CD48 consists of 220 amino acids and predicts a molecular mass of 25.60 kDa. It migrates as an approximately 42.34 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CD48 CD48 molecule [ Homo sapiens ]
• Official Symbol: CD48
• Synonyms: CD48; CD48 molecule; BCM1, CD48 antigen (B cell membrane protein) , CD48 molecule; CD48 antigen; BLAST; hCD48; mCD48; SLAMF2; TCT.1; BCM1 surface antigen; leukocyte antigen MEM-102; B-lymphocyte activation marker BLAST-1; CD48 antigen (B-cell membrane protein); BCM1; BLAST1; MEM-102
• Gene ID: 962
• mRNA Refseq: NM_001256030
• Protein Refseq: NP_001242959
• MIM: 109530
• UniProt ID: P09326