Active Recombinant Human CD40 protein, His/Fc-tagged, Biotinylated

• Cat.No.: CD40-202H
• Product Overview: Recombinant Human CD40(Leu 25 - Ser 645) fused with His and human IgG1 Fc fragment tag at C-terminal was expressed in HEK293, was enzymatically single-point biotinlabeled at C-terminus to retain its native conformational structure and bioactivity.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc&His
• Protein Length: 25-645 a.a.
• Predicted N Terminal: Glu 21
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Human CD40 Ligand, His Tag at 5 μg/mL ( 100 μl/well ) can bind Biotinylated Human CD40, His Tag & Fc Tag with a linear range of 0.2-1μg/mL.
• Molecular Mass: Has a calculated MW of 48 kDa. DTT-reduced Protein migrates as 55-65 kDa in SDS-PAGE due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg of the Biotinylated Human CD40, His Tag & Fc tag by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Applications: Has been used successfully for ELISA, western blotting, inhibition assay, pull-down, SPR, FACS, and affinity purification, together with anti-biotin antibody, streptavidin or avidin-conjugated probes.
• Storage: Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).

Gene Information

• Gene Name: CD40 CD40 molecule, TNF receptor superfamily member 5 [ Homo sapiens ]
• Official Symbol: CD40
• Synonyms: CD40; CD40 molecule, TNF receptor superfamily member 5; TNFRSF5, tumor necrosis factor receptor superfamily, member 5; tumor necrosis factor receptor superfamily member 5; Bp50; p50; CD40L receptor; CD40 type II isoform; B cell-associated molecule; B cell surface antigen CD40; B-cell surface antigen CD40; CD40 antigen (TNF receptor superfamily member 5); tumor necrosis factor receptor superfamily, member 5; nerve growth factor receptor-related B-lymphocyte activation molecule; CDW40; TNFRSF5; MGC9013
• Gene ID: 958
• mRNA Refseq: NM_001250
• Protein Refseq: NP_001241
• MIM: 109535
• UniProt ID: P25942
• Chromosome Location: 20q12-q13.2
• Pathway: Adaptive Immune System, organism-specific biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Asthma, organism-specific biosystem; Asthma, conserved biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem
• Function: enzyme binding; protein binding; receptor activity; signal transducer activity