Active Recombinant Human CD4 Protein (Lys 26 - Pro 396), Fc & Avi-tagged, Biotinylated

• Cat.No.: CD4-314H
• Product Overview: Recombinant Human CD4 Protein, Fc & Avi-tagged, Biotinylated is expressed from human 293 cells (HEK293). It contains AA Lys 26 - Pro 396 (Accession # P01730-1). Predicted N-terminus: Lys 26. This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag. The protein has a calculated MW of 69.7 kDa. As a result of glycosylation, the protein migrates as 80 kDa under reducing (R) condition, and 150 kDa under non-reducing (NR) condition (SDS-PAGE).

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&Fc
• Protein Length: Lys 26 - Pro 396
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized HIV GP120 (HIV), His Tag at 5 μg/mL (100 μL/well) can bind Recombinant Human CD4 protein, Fc & Avi-tagged, Biotinylated with a linear range of 0.5-8 ng/mL (QC tested).
• Molecular Mass: 69.7 kDa
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Notes: Please avoid repeated freeze-thaw cycles.
• Stability: No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: CD4 CD4 molecule [ Homo sapiens ]
• Official Symbol: CD4
• Synonyms: CD4; CD4 molecule; CD4 receptor; CD4 antigen (p55); CD4mut
• Gene ID: 920
• mRNA Refseq: NM_000616
• Protein Refseq: NP_000607
• MIM: 186940
• UniProt ID: P01730