Active Recombinant Rat CD4 protein, His-tagged

• Cat.No.: CD4-1075R
• Product Overview: Recombinant Rat CD4 protein(NP_036837.1)(Met1-Thr394), fused with His tag, was expressed in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: Met1-Thr394
• Form: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells.When 5 × 10E4 cells/well are added to ratCD4-His coated plates (1.25μg/mL and 100μL/well),approximately 20%-50% will adhere specifically after 30 minutes at 37℃.
• Molecular Mass: The recombinant rat Cd4 consists 378 amino acids and predicts a molecular mass of 42.5 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Cd4 Cd4 molecule [ Rattus norvegicus ]
• Official Symbol: CD4
• Synonyms: CD4; Cd4 molecule; T-cell surface glycoprotein CD4; CD4 antigen; W3/25 antigen; T-cell surface antigen T4/Leu-3; p55; W3/25
• Gene ID: 24932
• mRNA Refseq: NM_012705
• Protein Refseq: NP_036837