Active Recombinant Human CD3E Protein, His-Avi-tagged, Biotinylated

• Cat.No.: CD3E-2193H
• Product Overview: Biotinylated Human CD3 epsilon, His Tag, Avi Tag is expressed from HEK293 cells. It contains AA Asp 23 - Asp 126 (Accession # P07766-1). This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 23-126 a.a.
• Predicted N Terminal: Asp 23
• Form: Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
• Bio-activity: Immobilized Anti Human CD3e MAb at 2 ug/mL (100 uL/well) can bind Biotinylated Human CD3 epsilon, His Tag, Avi Tag with a linear range of 0.2-6 ug/mL.
• Molecular Mass: The protein has a calculated MW of 15.5 kDa. The protein migrates as 17-18 kDa under reducing condition (SDS-PAGE) due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Characteristic: Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: CD3E CD3e molecule, epsilon (CD3-TCR complex) [ Homo sapiens ]
• Official Symbol: CD3E
• Synonyms: CD3E; CD3e molecule, epsilon (CD3-TCR complex); CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell antigen receptor complex, epsilon subunit of T3; T3E; TCRE; FLJ18683
• Gene ID: 916
• mRNA Refseq: NM_000733
• Protein Refseq: NP_000724
• UniProt ID: P07766