Active Recombinant Human CD3D & CD3E Protein, His-Fc & Flag-Fc-tagged

• Cat.No.: CD3D & CD3E-1247H
• Product Overview: Recombinant extracellular domain (Met 1-Ala 105) of human CD3D (P04234) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; Recombinant extracellular domain (Met 1-Asp 126) of human CD3E (P07766)

Specification

• Species: Human
• Source: HEK293
• Tag: Fc&Flag&His
• Protein Length: 1-105;1-126 a.a.
• Predicted N Terminal: Phe 22 & Asp 23
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human CD3D & CD3E Heterodimer Protein at 10 μg/ml (100 μl/well) can bind OKT3-mIgG2a. The EC50 of OKT3-mIgG2a is 0.07-0.17 μg/ml.
• Molecular Mass: The recombinant heterodimer of human CD3D/CD3E comprises 670 (333+354) amino acids and has a calculated molecular mass of 77.5 (37.5+40) kDa. As a result of glycosylation, the apparent molecular mass of human CD3D/CD3E heterodimer is approximately 45-50 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >90 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: CD3D CD3d molecule, delta (CD3-TCR complex) [ Homo sapiens ]
• Official Symbol: CD3D
• Synonyms: CD3D; CD3d molecule, delta (CD3-TCR complex); CD3d antigen, delta polypeptide (TiT3 complex) , T3D; T-cell surface glycoprotein CD3 delta chain; CD3 delta; OKT3, delta chain; CD3 antigen, delta subunit; T-cell receptor T3 delta chain; CD3d antigen, delta
• Gene ID: 915
• mRNA Refseq: NM_000732
• Protein Refseq: NP_000723
• MIM: 186790
• UniProt ID: P04234
• Chromosome Location: 11q23
• Pathway: Adaptive Immune System, organism-specific biosystem; CXCR4-mediated signaling events, organism-specific biosystem; Chagas disease (American trypanosomiasis), organism-specific biosystem; Chagas disease (American trypanosomiasis), conserved biosystem; Cost
• Function: protein heterodimerization activity; receptor activity; transcription coactivator activity; transmembrane signaling receptor activity