Active Recombinant Human CD300C Protein, Fc-Tagged

• Cat.No.: CD300C-02H
• Product Overview: Mouse myeloma cell line, NS0-derived Recombinant Human CD300C Protein, Fc-Tagged

Specification

• Description: The CMRF35 antigen, which was identified by reactivity with a monoclonal antibody, is present on monocytes, neutrophils, and some T and B lymphocytes
• Source: Mouse myeloma cell line
• Species: Human
• Tag: Fc
• Form: Lyophilized
• Bio-activity: Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. Human T lymphocytes cultured for 72 hours with PHA were incubated for an additional 3 days in 96 well plates coated with 500 ng/mL anti‑CD3 and Recombinant Human CD300c Fc Chimera.
  The ED50 for this effect is 4-16 μg/mL.
  Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: 43.5 kDa (monomer)
• N-terminal Sequence Analysis: Met29
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 °C as supplied.
  1 month, 2 to 8 °C under sterile conditions after reconstitution.
  3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Gene Information

• Gene Name: CD300C CD300c molecule [ Homo sapiens (human) ]
• Official Symbol: CD300C
• Synonyms: CD300c; CLM6; CLM-6; CMRF35; IGSF16
• Gene ID: 10871
• mRNA Refseq: NM_006678.5
• Protein Refseq: NP_006669.1
• MIM: 606786
• UniProt ID: Q08708