Active Recombinant Human CD300 Molecule-Like Family Member F, Fc-tagged

• Cat.No.: CD300LF-5608H
• Product Overview: Recombinant Human CD300LFprotein was expressed in Murine myeloma cell line with the Fc part of humanIgG1 Pro100-Lys330 (C-terminus) and Tyr16-Leu155 (N-terminus).

Specification

• Cat. No.: CD300LF-5608H
• Description: CD300LF, also knownas CD300F, IREM-1, CLM-1, IgSF13, DIgR1, and MAIR-V, is a 50-60 kDaglycoprotein member of the immunoglobulin super-family. Human CD300LFconsists of a 137 amino acid (aa) extracellular domain (ECD) with one Ig-likeV-type domain, a 21 aa transmembrane segment, and a 113 aa cytoplasmic domainthat contains multiple immunoreceptor tyrosine-based inhibitory motifs(ITIMs) or ITIM-like sequences.
• Source: Mammalian cells
• Form: Lyophilized from a0.2 μm filtered solution in PBS.
• N-terminalSequence: Tyr16 & Thr20
• Molecular Weight: 42.4 kDa
• Activity: Measuredby its ability to stimulate human T cell proliferation. The ED50for this effect is typically 0.75-4.5 μg/mL. Optimal dilutions should bedetermined by each laboratory for each application.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• SDS-PAGE: 57-62kDa, reducing conditions
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstituteat 100 μg/mL in PBS
• Storage: Use a manualdefrost freezer and avoid repeated freeze-thaw cycles. 12 months from date ofreceipt, -20 to -70°C as supplied. 1 month, 2 to 8 °C under sterile conditionsafter reconstitution. 3 months, -20 to -70°C under sterile conditions after reconstitution.
• Protein length: 16-155 a.a.

Gene Information

• Gene Name: CD300LF CD300 molecule-likefamily member f [ Homo sapiens ]
• Official Symbol: CD300LF
• Synonyms: CD300LF; CD300molecule-like family member f; CLM1; NKIR; CLM-1; IREM1; CD300f; IREM-1;IgSF13; CMRF35-like molecule 1; NK inhibitory receptor; inhibitory receptorIREM1; CD300 antigen like family member F; CD300 antigen-like family memberF; immunoglobin superfamily member 13; CD300 molecule like family member f;immunoglobulin superfamily member 13; immune receptor expressed on myeloidcells 1; NKIR; UNQ3105/PRO10111; OTTHUMP00000070875; CD300f antigen
• Gene ID: 146722
• mRNA Refseq: NM_139018
• Protein Refseq: NP_620587
• MIM: 609807
• UniProt ID: Q8TDQ1
• Chromosome Location: 17q25.2
• Function: receptor activity