Active Recombinant Human CD28 protein, hIgG-His-tagged

Description :	CD28, also known as T-cell-specific surface glycoprotein CD28 isoform 1, is one of the proteins expressed on T cells that provide co-stimulatory signals required for T cell activation and survival. T cell stimulation through CD28 in addition to the T-cell receptor (TCR) can provide a potent signal for the production of various interleukins (IL-6 in particular). CD28/B7 interaction has been shown to prevent apoptosis of activated T cells via the up-regulation of bcl-XL. It ligation has also been shown to regulate Th1/Th2 differentiation. Recombinant human CD28, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Source :	Insect cell
Species :	Human
Tag :	His&Fc
Form :	Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol.
Bio-activity :	Measured by its binding ability in a functional ELISA with Human CD80. The ED50 range ≤ 10ug/ml.
Molecular Mass :	42.4 kDa
Protein length :	19-152aa
AA Sequence :	NKILVKQ SPMLVAYDNA VNLSCKYSYN LFSREFRASL HKGLDSAVEV CVVYGNYSQQ LQVYSKTGFN CDGKLGNESV TFYLQNLYVN QTDIYFCKIE VMYPPPYLDN EKSNGTIIHV KGKHLCPSPL FPGPSKP
Endotoxin :	< 1 EU per 1ug of protein (determined by LAL method).
Purity :	> 90%
Applications :	SDS-PAGE, Bioactivity
Storage :	Can be stored at 2 centigrade to 8 centigrade for 1 week. For long term storage, aliquot and store at -20 centigrade to -80 centigrade. Avoid repeated freezing and thawing cycles.
Concentration :	1mg/ml

Gene Name	CD28 CD28 molecule [ Homo sapiens (human) ]
Official Symbol	CD28
Synonyms	T-cell-specific surface glycoprotein CD28 isoform 1; CD28; Tp44
Gene ID	940
mRNA Refseq	NM_006139
Protein Refseq	NP_006130
MIM	186760
UniProt ID	P10747