Active Recombinant Human CD274 protein(Met1-Thr239), hFc-tagged

• Cat.No.: CD274-2330H
• Product Overview: Recombinant Human CD274 protein(NP_054862.1)(Met1-Thr239), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-Thr239
• similar: CD274
• Alias: CD274; CD274 molecule; CD274 antigen , PDCD1LG1, programmed cell death 1 ligand 1; programmed cell death 1 ligand 1; B7 homolog 1; B7 H; B7 H1; B7H1; PD L1; PDL1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1; B7-H; PD-L1; PDCD1L1; PDCD1LG1; MGC142294; MGC142296
• Conjugate: C-hFc
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bioactivity: 1. Immobilized human PD-1 at 2 μg/ml (100 μl/well) can bind human B7-H1, the EC50 of human B7-H1 is 150-600 ng/mL. 2. Using the Octet RED System, the affinity constant (Kd) of PD-L1 Protein, Human, Recombinant (Fc Tag) bound to Atezolizuma was 0.5 nM.
• MolecularMass: The recombinant mature human B7-H1/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 459 amino acids and predicts a molecular mass of 52 kDa. As a result of glycosylation, the human B7-H1/Fc monomer migrates as an approximately 65-70 kDa protein in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• GeneName: CD274 CD274 molecule [ Homo sapiens ]
• GeneNameWeblink: 29126
• OfficialSymbol: CD274
• OfficialSymbolWeblink: 29126
• GeneID: 29126
• GeneIDWeblink: 29126
• mRNARefseq: NM_014143
• mRNARefseqWeblink: NM_014143.3
• ProteinRefseqWeblink: NP_054862.1
• MIMWeblink: 605402
• UniProtID: Q9NZQ7
• UniProtIDWeblink: Q9NZQ7

Gene Information

• Synonyms: CD274; CD274 molecule; CD274 antigen , PDCD1LG1, programmed cell death 1 ligand 1; programmed cell death 1 ligand 1; B7 homolog 1; B7 H; B7 H1; B7H1; PD L1; PDL1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1; B7-H; PD-L1; PDCD1L1; PDCD1LG1; MGC142294; MGC142296
• MIM: 605402