Active Recombinant Human CCL2 Protein, His-tagged

• Cat.No.: CCL2-11H
• Product Overview: Recombinant human CCL2/JE/MCP-1 (24-99aa, 82aa), fused to His-tag at C-terminus, was expressed in HEK293 and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 24-99
• Form: Liquid
• Bio-activity: Measured its ability to chemoattract using THP-1 human acute monocytic leukemia cells. The ED50 range ≤ 30 ng/mL.
• Molecular Mass: 9.5 kDa
• AA Sequence: QPDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAKEICADPKQKWVQDSMDHLDKQTQTPKT
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 90% by SDS-PAGE
• Applications: SDS-PAGE, Bioactivity
• Storage: Can be stored at 2 to 8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/mL (determined by Absorbance at 280nm)
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Gene Information

• Gene Name: CCL2 C-C motif chemokine ligand 2 [ Homo sapiens (human) ]
• Official Symbol: CCL2
• Synonyms: CCL2; C-C motif chemokine ligand 2; HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30; C-C motif chemokine 2; chemokine (C-C motif) ligand 2; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; monocyte chemotactic protein 1; monocyte secretory protein JE; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); small inducible cytokine subfamily A (Cys-Cys), member 2; small-inducible cytokine A2
• Gene ID: 6347
• mRNA Refseq: NM_002982
• Protein Refseq: NP_002973
• MIM: 158105
• UniProt ID: P13500