Recombinant Rat Chemokine (C-C Motif) Ligand 2

• Cat.No.: Ccl2-55R
• Product Overview: Recombinant Rat Monocyte Chemotactic Protein-1 produced in E.Coli is a non-glycosylated, Polypeptide chain containing 125 amino acids and having a molecular mass of 14092 Dalton. The MCP-1 is purified by proprietary chromatographic techniques.

Specification

• Species: Rat
• Source: E.coli
• Tag: Non
• Description: Chemokine (C-C motif) ligand 2 (CCL2) is a small cytokine belonging to the CC chemokine family that is also known as monocyte chemotactic protein-1 (MCP-1). It is found at the site of tooth eruption and bone degradation. In the bone, CCL2 is expressed by mature osteoclasts and osteoblasts and is under the control of nuclear factor κB (NFκB). CCL2 recruits immune cells, such as monocytes, to sites of tissue injury and infection. This chemokine is produced as a protein precursor containing signal peptide of 23 amino acids and a mature peptide of 76 amino acids. It is a monomeric polypeptide, with a molecular weight of approximately 13 kDa.
• Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Gln-Pro-Asp-Ala-Val.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Purity: Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: The protein was lyophilized from a concentrated (1mg/ml) sterile solution containing no additives.
• Solubility: It is recommended to reconstitute the lyophilized Monocyte Chemotactic Protein-1 in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
• Specific Activity: The Biological activity is calculated by its ability to chemoattract total rat neutrophils cells using 10.0-100.0 ng/ml.
• Stability: Lyophilized MCP-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL2 should be stored at 4°Cbetween 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: Ccl2 chemokine (C-C motif) ligand 2 [ Rattus norvegicus ]
• Synonyms: Ccl2; chemokine (C-C motif) ligand 2; MCP-1; Scya2; Sigje;monocyte chemotactic protein 1; small inducible cytokine A2; monocyte chemoattractant protein-1; Small inducible gene JE
• Gene ID: 24770
• mRNA Refseq: NM_031530
• Protein Refseq: NP_113718
• MIM: 112266
• UniProt ID: P14844
• Chromosome Location: 10q26
• Pathway: Chemokine signaling pathway; Cytokine-cytokine receptor interaction
• Function: CCR2 chemokine receptor binding; G-protein-coupled receptor binding; heparin binding; protein binding