Active Recombinant Human CCL2 Protein (76 aa)

• Cat.No.: CCL2-080C
• Product Overview: Recombinant Human CCL2 Protein without tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Protein Length: 76
• Description: CCL2, also known as monocyte chemotactic and activating factor (MCAF), was initially purified independently by two groups based on its ability to chemoattract monocytes. Subsequent to its cloning and sequencing, it became evident that this protein is also identical to the product of the human JE gene. The JE gene, originally identified in mouse fibroblasts, is a plateletderived growth factor (PDGF)inducible gene. The human CCL2 cDNA encodes a 99 amino acid residue precursor protein with a 23 residue hydrophobic signal peptide that is cleaved to generate the 76 residue mature protein. Natural CCL2 is heterogeneous in size due to the addition of Olinked carbohydrates and sialic acid residues. In addition to fibroblasts; tumor cells, smooth muscle cells, endothelial cells, and mononuclear phagocytes can also produce CCL2 either constitutively or upon stimulation by various stimuli. CCL2 is a member of the β (CC) subfamily of chemokines. Recently, the existence of MCP2 and MCP3 with 62% and 73% amino acid identity respectively, to CCL2 have been reported.
• Form: Sterile Filtered White lyophilized (freeze-dried) powder.
• Bio-activity: Measured by its ability to chemoattract human CCR2A transfected BaF3 mouse pro-B cells. The ED50 for this effect is typically 10-60 ng/mL, corresponding to a Specific Activity of ＞1.7 × 10^4 IU/mg.
• Molecular Mass: 8.6 kDa, a single non-glycosylated polypeptide chain containing 76 amino acids.
• AA Sequence: QPDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAKEICADPKQKWVQDSMDHLDKQTQTPKT
• Endotoxin: Less than 1 EU/mg of rHuMCP-1/MCAF/CCL2 as determined by LAL method.
• Purity: >96% by SDS-PAGE and HPLC analyses.
• Storage: This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 to -70 centigrade. Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2mm filtered concentrated solution in 20mM PB, pH 7.4, 100mM NaCl.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 centigrade. Further dilutions should be made in appropriate buffered solutions.

Gene Information

• Gene Name: CCL2 chemokine (C-C motif) ligand 2 [ Homo sapiens ]
• Official Symbol: CCL2
• Synonyms: CCL2; chemokine (C-C motif) ligand 2; SCYA2, small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je); C-C motif chemokine 2; GDCF 2; HC11; MCAF; MCP 1; MCP1; MGC9434; monocyte chemoattractant protein 1; monocyte chemotactic and activating factor; monocyte chemotactic protein 1; homologous to mouse Sig je; monocyte secretory protein JE; small inducible cytokine subfamily A (Cys Cys); member 2; SMC CF; small-inducible cytokine A2; monocyte chemoattractant protein-1; small inducible cytokine subfamily A (Cys-Cys), member 2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30
• Gene ID: 6347
• mRNA Refseq: NM_002982
• Protein Refseq: NP_002973
• MIM: 158105
• UniProt ID: P13500