Active Recombinant Human Cardiotrophin 1, GST-tagged

• Cat.No.: CTF1-184H

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Description: The CCAAT-box Binding Protein 1 (CTF1, also called NF-I or NF-1) is a proline-rich transcription activator. It selectively activates transcription of genes that contain GCCAAT consensus sequence in promoter region, including human Ha-Ras, alpha-globin, hsp70 and many other cellular and viral genes. Cloning and functional characterization of the protein revealed that CTF1 contains a N-terminal DNA binding domain and a C-terminal proline-rich activation domain. CTF1 was shown to interact with the general transcription factor IIB (TFIIB) and facilitate the recruitment of TFIIB to TBP-DNA complexes, with the forkhead thyroid transcription factor TTF2 to regulate hormonedependent transcription of thyroperoxidase gene, as well as other receptor proteins to synergize hormone receptor-responsive activation. The proline-rich activation domain of human CTF1 protein (amino acids 399-499) is isolated from an E. coli strain that carries the coding sequence of the fused protein under the control of a T7 promoter. GST-CTF1 has been used for protein-protein interaction assays.
• Usage: For in vitro use only.
• Form: Liquid. Supplied in 20 mM Tris-HCl pH 8.0, 100 mM KCl, 0.2 mM EDTA, 1 mM DTT and 20% glycerol.
• Activity: 100 ng are sufficient for a protein-protein interaction assay.
• Purity: > 95% by SDS-PAGE.
• Storage: Quality guaranteed for 12 months, Store at -80℃. Avoid freeze / thaw cycles.

Gene Information

• Gene Name: CTF1 cardiotrophin 1 [ Homo sapiens ]
• Synonyms: CTF1; cardiotrophin 1; CT1; CT-1; cardiophin 1; Cardiotrophin-1
• Gene ID: 1489
• mRNA Refseq: NM_001142544
• Protein Refseq: NP_001136016
• MIM: 600435
• UniProt ID: Q16619
• Chromosome Location: 16p11.2
• Pathway: Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway
• Function: cytokine activity; leukemia inhibitory factor receptor binding