Active Recombinant Human BTN3A2 protein, His/Avi-tagged, Biotinylated

• Cat.No.: BTN3A2-051H
• Product Overview: Biotinylated Recombinant Human BTN3A2(Gln30-Trp248) protein, fused to His/Avi tag at the C-terminus, was expressed in HEK293 cells .

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Gln30-Trp248
• Description: BTN3A2 (Butyrophilin subfamily 3 member A2; also BTF3 and BT3.2) is a 36 kDa (predicted) glycoprotein, member of the BTN family, Ig Superfamily of molecules. It is postulated to be expressed on immune-related cells, as it has a structural similarity to MHC and CD80/CD86 molecules. Mature human BTN3A2 is a 305 amino acid (aa) type I transmembrane protein. It contains a 219 aa extracellular region with one V-type Ig-like domain and a 65 aa cytoplasmic tail. The cytoplasmic region undergoes phosphorylation on two serines. There are three potential splice forms. A rodent counterpart to BTN3A2 has not been reported. BTN3A2 mRNA over‑expression was associated with a good prognosis in relation to disease-free and overall survival in a cohort of 55 epithelial ovarian cancer (EOC) patients (1). Another study in a larger cohort of 199 high-grade EOC patients further confirmed that the protein expression of BTN3A2 in ovarian cancer tissues is positively correlated with the intraepithelial infiltration of CD4+ and CD8+ T cells (2), suggesting that BTN3A2 was a co-stimulatory molecule to modulate the infiltration of immune cells and thus the anti-cancer immunity. In consistent with previous publications, our in-house studies on BTN3A2 showed that BTN3A2 co-stimulated anti‑CD3 induced IFN-gamma secretion on CD3+ cells.
• Form: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Bio-activity: Measured by its binding ability in a functional ELISA. When Sheep Anti-Human BTN3A1/2 Antigen Affinity-purified Polyclonal Antibody is immobilized at 1.0 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human BTN3A2 His-tag Avi-tag that produces 50% of the optimal binding response is approximately 15-75 ng/mL.
• Molecular Mass: 27-37 kDa, under reducing conditions
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Applications: Bioactivity
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 200 μg/mL in PBS.

Gene Information

• Gene Name: BTN3A2 butyrophilin, subfamily 3, member A2 [ Homo sapiens ]
• Official Symbol: BTN3A2
• Synonyms: BTN3A2; butyrophilin, subfamily 3, member A2; butyrophilin subfamily 3 member A2; butyrophilin protein; BTF4; BT3.2; BT3.3; FLJ40011
• Gene ID: 11118
• mRNA Refseq: NM_001197246
• Protein Refseq: NP_001184175
• MIM: 613594
• UniProt ID: P78410