Active Recombinant Human BTLA protein, hFc-tagged

• Cat.No.: BTLA-2928H
• Product Overview: Recombinant Human BTLA protein(NP_001078826.1)(Met1-Thr134), fused with hFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-Thr134
• Form: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1.Measured by its binding ability in a functional ELISA. 2.Immobilized human BTLA-Fc at 10μg/mL (100μL/well) can bind biotinylated mouse HVEM-Fch, the EC50 of biotinylated mouse HVEM-Fch is 6-70ng/mL.
• Molecular Mass: The recombinant human BTLA comprises 342 amino acids and has a predicted molecular mass 38.8 kDa. As a result of glycosylation, it migrates as an approximately 53.7 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: BTLA B and T lymphocyte associated [ Homo sapiens ]
• Official Symbol: BTLA
• Synonyms: BTLA; B and T lymphocyte associated; B- and T-lymphocyte attenuator; BTLA1; CD272; B and T lymphocyte attenuator; B- and T-lymphocyte-associated protein; FLJ16065; MGC129743
• Gene ID: 151888
• mRNA Refseq: NM_001085357
• Protein Refseq: NP_001078826
• MIM: 607925
• UniProt ID: Q7Z6A9