Active Recombinant Human BTC Protein (Asp32-Gln118), GMP Grade, Animal-Free

• Cat.No.: BTC-02HG
• Product Overview: GMP Recombinant Human BTC Protein, Asp32-Gln118, with an N-terminal Met was expressed in E. coli and produced using non-animal reagents in an animal-free laboratory under cGMP guidelines.

Specification

• Species: Human
• Source: E.coli
• Protein Length: Asp32-Gln118
• Description: This gene encodes a member of the epidermal growth factor (EGF) family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the secreted growth factor. A secreted form and a membrane-anchored form of this protein bind to multiple different EGF receptors. This protein promotes pancreatic cell proliferation and insulin secretion, as well as retinal vascular permeability. Mutations in this gene may be associated with type 2 diabetes in human patients.
• Bio-activity: Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.100-1.50 ng/mL.
• Molecular Mass: Predicted Molecular Mass: 9.9 kDa
  SDS-PAGE: 12-15 kDa, under reducing conditions.
• N-terminal Sequence Analysis: Met-Asp32
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: > 97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Reconstitution: Reconstitute at 100-500 μg/mL in PBS.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: BTC betacellulin [ Homo sapiens ]
• Official Symbol: BTC
• Synonyms: BTC; betacellulin; probetacellulin
• Gene ID: 685
• mRNA Refseq: NM_001729
• Protein Refseq: NP_001720
• MIM: 600345
• UniProt ID: P35070