Active Recombinant Human Bone Morphogenetic Protein 5

• Cat.No.: BMP5-27H
• Product Overview: Recombinant Human BMP5 (Ala317­His454) was expressed in CHO Cells.

Specification

• Species: Human
• Source: CHO
• Tag: Non
• Description: This gene encodes a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. These proteins are synthesized as prepropeptides, cleaved, and then processed into dimeric proteins. This protein may act as an important signaling molecule within the trabecular meshwork and optic nerve head, and may play a potential role in glaucoma pathogenesis. This gene is differentially regulated during the formation of various tumors.
• Molecular Mass: This protein has a calculated molecular mass of 15.6 kDa, migrates as an approximately 18-23 kDa band in SDS-PAGE under reducing conditions as predicted.
• Predicted N terminal: Ala317
• Formulation: Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
• Purity: >95%, by SDS­PAGE under reducing conditions and visualized by silver stain.
• Activity: Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is typically 0.2-1.2 µg/mL.
• EndotoxinLevel: <0.01 EU per 1 μg of the protein by the LAL method.
• Reconstitution: Reconstitute at 100 μg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin.
• Storage: 12 months from date of receipt, ­20 to ­70 °C as supplied. Avoid repeated freeze­thaw cycles.
• OfficialSymbol: BMP5

Gene Information

• Gene Name: BMP5 bone morphogenetic protein 5 [ Homo sapiens ]
• Synonyms: BMP5; bone morphogenetic protein 5; MGC34244; BMP-5; OTTHUMP00000016650
• Gene ID: 653
• mRNA Refseq: NM_021073
• Protein Refseq: NP_066551
• MIM: 112265
• UniProt ID: P22003
• Chromosome Location: 6p12.1
• Pathway: TGF-beta signaling pathway
• Function: BMP receptor binding; cytokine activity; growth factor activity