Active Recombinant Human BLK protein, GST-tagged

• Cat.No.: BLK-953H
• Product Overview: Recombinant Human BLK protein(NP_001706.2)(Met1-Pro505), fused with GST tag, was expressed in Insect Cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST
• Protein Length: Met1-Pro505
• Form: Supplied as sterile 20mM Tris, 500mM NaCl, 5mM GSH, pH 7.4Please contact us for any concerns or special requirements.Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: The specific activity was determined to be 17.4 nmol/min/mg using Poly(Glu,Tyr)4:1 peptide as substrate.
• Molecular Mass: The recombinant human BLK/GST chimera consists of 730 amino acids and migrates as an approximatly 84 kDa band as predicted in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 88 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: BLK B lymphoid tyrosine kinase [ Homo sapiens ]
• Official Symbol: BLK
• Synonyms: BLK; B lymphoid tyrosine kinase; tyrosine-protein kinase Blk; MGC10442; p55-Blk; b lymphocyte kinase; BLK nonreceptor tyrosine kinase; MODY11
• Gene ID: 640
• mRNA Refseq: NM_001715
• Protein Refseq: NP_001706
• MIM: 191305
• UniProt ID: P51451