Active Recombinant Human BID protein

Source :	E. coli
Species :	Human
Tag :	Non
Form :	Lyophilized from sterile 40mM Tris, 150mM NaCl, pH 8.0Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Bio-activity :	Immobilized Human BID (Cat:10468-HNCE) at 2 μg/mL (100 μL/well) can bind Human Bcl-XL His (Cat:10455-H08E), the EC50 of Human Bcl-XL His (Cat:10455-H08E) is 15-80 ng/mL.
Molecular Mass :	The recombinant human BID consists of 197 amino acids and migrates as an approximately 22 kDa band in SDS-PAGE under reducing conditions as predicted.
Protein length :	Met1-Asp195
Purity :	> 90 % as determined by SDS-PAGE
Storage :	Samples are stable for up to twelve months from date of receipt at -20°C to -80°C Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution :	It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Name	BID BH3 interacting domain death agonist [ Homo sapiens ]
Official Symbol	BID
Synonyms	BID; BH3 interacting domain death agonist; BH3-interacting domain death agonist; p22 BID; BID isoform Si6; BID isoform L(2); BID isoform ES(1b); desmocollin type 4; apoptic death agonist; Human BID coding sequence; FP497; MGC15319; MGC42355;
Gene ID	637
mRNA Refseq	NM_001196
Protein Refseq	NP_001187
MIM	601997
UniProt ID	P55957