Active Recombinant Human Baculoviral IAP Repeat-Containing 2, AVI-tagged

• Cat.No.: BIRC2-3104H
• Product Overview: Recombinant Human BIRC2 (Glu 144-Leu 356) with a AVI tag at C-terminus and additonal two amino acids (Gly & Pro) at the N-terminus, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Avi
• Protein Length: 144-356 a.a.
• Description: Baculoviral IAP repeat-containing protein 2, also known as Inhibitor of apoptosis protein 2, HIAP2, c-IAP1, RING finger protein 48, and BIRC2, is a member of the IAP family. The inhibitor of apoptosis (IAP) proteins are a family of anti-apoptotic regulators found in viruses and metazoans. The UBA (ubiquitin-associated) domain of IAPs is located between the BIR (baculovirus IAP repeat) domains and the CARD (caspase activation and recruitment domain) or the RING (really interesting new gene) domain of c-IAP1 and c-IAP2 or XIAP (X-linked IAP) respectively. c-IAP1 contains three BIR repeats, one CARD domain and one RING-type zinc finger. c-IAP1 and c-IAP2 are recruited to tumor necrosis factor receptor 1 (TNFR1) associated complexes where they can regulate receptor-mediated signaling. Both c-IAP1 and c-IAP2 have been implicated in TNFalpha-stimulated NF-kappaB activation. Treatment of cells with IAP antagonists leads to proteasomal degradation of c-IAP1 and c-IAP2. Deletion or mutation of the UBA domain decreases this degradation, probably by diminishing the interaction of the c-IAPs with the proteasome. Ubiquitin binding may be an important mechanism for rapid turnover of auto-ubiquitinated c-IAP1 and c-IAP2.
• Formulation: Lyophilized from sterile 10mM Tris, 5% glycerol, 0.5mM EDTA, 5mM DTT, pH 7.5
• Purity: > 92 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Bio-activity: Measured by its ability to inhibit DEVD-AFC cleavage activity in cell extracts activated by addition of cytochrome c and dATP.
• Stability: Samples are stable for up to twelve months from date of receipt at -70°C.
• Predicted N terminal: Gly
• Molecular Mass: The recombinant human cIAP1 ( Glu 144-Leu 356 ) consists of 230 amino acids and has a calculated molecular mass of 26.5 kDa as estimated by SDS-PAGE under reducing conditions.
• Storage: Store it under sterile conditions at -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: BIRC2 baculoviral IAP repeat-containing 2 [ Homo sapiens ]
• Synonyms: BIRC2; baculoviral IAP repeat-containing 2; IAP homolog B; IAP-2; NFR2-TRAF signalling complex protein; RING finger protein 48; TNFR2-TRAF-signaling complex protein 2; apoptosis inhibitor 1; baculoviral IAP repeat-containing protein 2; inhibitor of apoptosis protein 2; API1; MIHB; HIAP2; RNF48; cIAP1; Hiap-2; c-IAP1
• Gene ID: 329
• mRNA Refseq: NM_001166
• Protein Refseq: NP_001157
• MIM: 601712
• UniProt ID: Q13490
• Chromosome Location: 11q22
• Pathway: Apoptosis; Focal adhesion; NOD-like receptor signaling pathway;Pathways in cancer; Small cell lung cancer; Ubiquitin mediated proteolysis; Apoptosis
• Function: metal ion binding; protein N-terminus binding; protein binding; zinc ion binding