Active Recombinant Human ANGPT2 protein, His & Avi-tagged, Biotinylated

• Cat.No.: ANGPT2-524HB
• Product Overview: Biotinylated Recombinant Human ANGPT2 protein (NP_001138.1) (Lys275-Phe496) was expressed in HEK293, fused with a c-terminal AVI tagged polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: His&Avi
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Immobilized Recombinant Human Tie2 / CD202b / TEK Protein (ECD, His Tag) at 2 μg/ml (100 μl/well) can bind Recombinant Human Angiopoietin-2 Protein (AVI & His Tag), Biotinylated, the EC50 is 300-900 ng/mL.
• Molecular Mass: The recombinant human ANGPT2 consists of 248 amino acids and predicts a molecular mass of 28.72 kDa. It migrates as an approximately 30.74 kDa band in SDS-PAGE under reducing conditions.
• Protein length: Lys275-Phe496
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ANGPT2 angiopoietin 2 [ Homo sapiens ]
• Official Symbol: ANGPT2
• Synonyms: ANGPT2; angiopoietin 2; angiopoietin-2; Ang2; ANG-2; Tie2-ligand; angiopoietin-2B; angiopoietin-2a; ANG2; AGPT2
• Gene ID: 285
• mRNA Refseq: NM_001118887
• Protein Refseq: NP_001112359
• MIM: 601922
• UniProt ID: O15123