Recombinant Human ANGPT2 protein, His-tagged

• Cat.No.: ANGPT2-531H
• Product Overview: Recombinant Human ANGPT2 (O15123) aa. (Lys24~Leu165) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 18kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Lys24~Leu165
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >92%
• Characteristic: The isoelectric point is 5.4.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Gene Information

• Gene Name: ANGPT2 angiopoietin 2 [ Homo sapiens (human) ]
• Official Symbol: ANGPT2
• Synonyms: ANGPT2; angiopoietin-2; ANG-2; Tie2-ligand; angiopoietin-2B; angiopoietin-2a; angiopoietin 2; ANG2; AGPT2
• Gene ID: 285
• mRNA Refseq: NM_001118887.1
• Protein Refseq: NP_001112359.1
• UniProt ID: O15123