Active Recombinant Human ALPPL2 protein, His-Avi-tagged, Biotinylated

• Cat.No.: ALPPL2-2862H
• Product Overview: Biotinylated Recombinant Human ALPPL2 protein(Ile 20-Asp 503), fused with His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Ile 20-Asp 503
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris,150 mM NaCl,pH7.5 with trehalose as protectant.
• Bio-activity: Immobilized Biotinylated Human ALPG / ALPPL2 Protein,His,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Anti-ALPG / ALPPL2 with a linear range of 0.1-4 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)The protein has a calculated MW of 56.2 kDa. The protein migrates as 60-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ALPPL2 alkaline phosphatase, placental-like 2 [ Homo sapiens ]
• Official Symbol: ALPPL2
• Synonyms: ALPPL2; alkaline phosphatase, placental-like 2; alkaline phosphatase, placental-like; ALP-1; Nagao isozyme; germ cell alkaline phosphatase; alkaline phosphatase Nagao isozyme; placental-like alkaline phosphatase; testicular and thymus alkaline phosphatase; ALPG; GCAP; ALPPL
• Gene ID: 251
• mRNA Refseq: NM_031313
• Protein Refseq: NP_112603
• MIM: 171810
• UniProt ID: P10696